GSM2781595: HL-48 and HL-58 Xylose Rep2; Halomonas sp. HL48; Halomona... - SRA

SRX3181551: GSM2781595: HL-48 and HL-58 Xylose Rep2; Halomonas sp. HL48; Halomonas sp. HL58; RNA-Seq
6 ABI_SOLID (AB 5500xl Genetic Analyzer) runs: 56.1M spots, 2.8G bases, 1.6Gb downloads

Submitted by: NCBI (GEO)

Study: Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture

PRJNA406985 • SRP117464 • All experiments • All runs

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Testing of response of hetertrophic partners (Halomonas sp. HL-48 and Marinobacter sp. HL-58) to both axenic and co-culture growth using both glucose and xylose Overall design: Both Halomonas sp. HL-48 and Marinobacter sp. HL-58 were grown either on glucose axenically or in co-culture and on xylose in co-culture. Halomonas HL-48 was also grown on xylose axenically. Transcriptomic data was collected under each condition and used to determine each organisms response to the other under both competitive and commensal conditions.

Sample: HL-48 and HL-58 Xylose Rep2

SAMN07637253 • SRS2510281 • All experiments • All runs

Organism: Halomonas sp. HL48

Library:

Instrument: AB 5500xl Genetic Analyzer

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: RNA was extracted using Trizol following by DNAseI treatment using Qiagen RNAseay mini column Template cDNA was prepared using the Applied Biosystems SOLiD® Total RNA-Seq Kit (Life Technologies, Carlsbad, CA) according to manufacturer's protocol. Sequencing was carried using the SOLiDTM 5500XL protocol (Life Technologies).

Experiment attributes:

GEO Accession: GSM2781595

Links:

NCBI link: NCBI Entrez (gds)

Runs: 6 runs, 56.1M spots, 2.8G bases, 1.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR6031305	9,321,264	466.1M	274.1Mb	2017-09-14
SRR6031306	9,524,234	476.2M	282Mb	2017-09-14
SRR6031307	9,377,697	468.9M	272.7Mb	2017-09-14
SRR6031308	9,503,487	475.2M	275.2Mb	2017-09-14
SRR6031309	9,316,090	465.8M	270.5Mb	2017-09-14
SRR6031310	9,100,053	455M	264.2Mb	2017-09-14

ID:: 4483504

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